A variety of strategies could also be used for assortment of oral samples. Saliva may be dropped onto FTA® paper as for blood samples, although clean saliva is often a poor supply of DNA. Swabs of both gums or cheeks are preferable sources of DNA, as swabbing or brushing detaches large numbers of cells.
Bird blood incorporates an extreme amount of DNA for even the smallest punched discs to be used immediately in PCR reactions. For instance six blood samples from Australian pelicans have been processed by methodology #4 and three of the 1 mm discs have been positioned into 50 μl water and heated to 90°C for 10 minutes to launch much of the DNA. The three remaining discs had been used instantly within the PCR as is usual apply with mammalian samples.
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B) Phenol extracted DNA from degraded samples and PCR amplification after purification using FTA paper. Digestion of DNA from blood (1â4) and tissue (5â9) samples on FTA paper. Samples had been crested pigeon , rooster , cow , sleepy lizard , Mallee fowl , frog , yabbie , abalone and blue crab . Samples have been processed, and digested overnight with AluI Fish blood samples.
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